Webinar Objectives: 

• Understand the Pre- & Post Transplant Antibody Identification in cases of Haplo Haematopoetic Stem Cell Transplant.
• Highlight the importance of monitoring.
• Discuss clinical case studies demonstrating the impact of antibody identification and monitoring on patient outcomes.

Speakers: Dr. Anil Handoo, Principal Director, Hospital Laboratory Services & Transfusion Medicine, & Principal Director Academics & Research - Max Healthcare Institute Limited & associated hospitals.

Objective:

• To discuss complex cases in immunhematology, focusing on the application of special reagents and methodologies to solve diagnostic and transfusion challenges.
• It presents two clinical cases that illustrate typical situations. The importance of techniques such as adsorption, elution and the use of specialized reagents (such as chloroquine and ZZAP) to characterize antibodies and guarantee safe transfusions, even in complex scenarios, is also emphasized.

Speaker: Regina Cardoso, MSc

Objective:

• To explore the variants of the RhD phenotype and how molecular alterations affect serological tests. It details types of mutations (deletions, insertions, gene conversions and single nucleotide variants), which can cause changes in RhD protein expression.
• These changes result in phenotypes such as D negative, Del, weak D and partial D, with different levels of antigenic expression and clinical impact. The importance of genetic and molecular understanding to improve diagnosis and safe transfusions is emphasized.

Speaker: Dr. Carine Prisco Arnoni

Objective:

• RhCE variants can complicate routine serology and require detailed investigation to determine the clinical significance of antibodies. Serological and molecular techniques are essential for the detection and management of RhCE variants.

Speaker: Dr. Tatiane Vendrame

Objective:

• The importance of molecular genotyping in clinical immunohematology, in diagnosis, transfusion management, and prevention of alloimmunization.
• It explores how genotyping techniques, such as next generation sequencing (NGS), overcome the limitations of serological tests, especially in cases of frequent transfusions.
• Practical examples demonstrate how genotyping improves transfusion compatibility and reduces complications, and the impact of genotyping on improving the safety and efficacy of transfusion medicine is emphasized.

Presenter: Lilian Castilho, PhD

Objective:

• Molecular techniques used to determine blood group alleles, emphasizing their application in immunohematology.
• Techniques such as PCR, PCR-RFLP, next generation sequencing and microarrays are highlighted for their efficiency in identifying clinically relevant alleles and rare variants.
• The importance of these tools to personalize and optimize transfusion management, promoting greater patient safety, is highlighted.

Presenter: Carine Prisco Arnoni

Objective:

• Antibodies of Undetermined Specificity (AUS) are antibodies or reactions that remain unexplained after excluding common antibodies, usually identified in irregular antibody screening (IAP) tests.
• Identification includes the use of extended phenotyping, different panels and chemical treatments (e.g. chloroquine, enzymes) to clarify specificities, as well as techniques such as adsorption/elution.

Presenter: Regina Cardoso , MSc

Objective:

• Solid phase platelet crossmatch has an application, methodology and clinical cases of Platelet Refractoriness or inadequate platelet increase after transfusions, usually associated with antibodies against platelet antigens (HLA, HPA).
• Detection techniques include the use of methods such as flow cytometry, PIFT, MAIPA and solid phase methodology (Capture-P), the latter used to detect antiplatelet antibodies and transfusion compatibility.
• Examples include strategies for refractory patients, the use of desensitization protocols and slow transfusions of incompatible platelets in critical cases.

Presenter: Juliana Pires Marafon Franz, MD, PhD

Isohemagglutinin tests (anti-A and anti-B) are antibodies that can be IgM, IgG or IgA, all of which have significant clinical potential and can cause intra- and extravascular hemolysis. ABO incompatibility (major or minor) can reduce the effectiveness of the transfusion and cause adverse reactions such as acute hemolysis. Practical examples demonstrate the seriousness of incompatible transfusions and the importance of prophylactic strategies. Example of local validation using automation to determine critical titers and define PC selection protocols. Presenter: Ana Paula Rocha Diniz Zanelli

Objective: This article discusses Platelet Crossmatch, an essential method for platelet compatibility in refractory patients. The technique is used to identify compatible platelets in alloimmunized patients. The method is based on the solid phase (Capture-P®), which detects IgG antibodies against platelets. It highlights the importance of standardized methods to improve the management of refractory patients and optimize clinical results. Speaker: Cristina Altobeli de Brito

Objective: To address RhD variants and the importance of their serological detection to guide molecular investigations. It explains how mutations such as SNPs, deletions and gene conversions result in variants such as partial D, weak D and Del. These alterations can cause differences in epitope expression and antisera reactivity. It includes examples of discrepancies in RhD typing and molecular characterization, such as a donor with a CDe haplotype and a patient with myelodysplastic syndrome. The importance of standardized methods and knowledge of variants for safe transfusion practices and accurate diagnoses is reinforced. Speaker: Dr. Tatiane de Paula Vendrame

Objective: A study of RhCE variants is presented, covering detection, classification and clinical significance. RhCE variants are detailed as variant proteins due to genetic polymorphisms, which can affect the expression of RhCE antigens such as C, c, E and e. Weakened expression, partial antigens and the absence of high-frequency antigens are mentioned. The need for greater knowledge of these variants to improve transfusions and blood compatibility in chronically transfused patients is emphasized. Speaker: Dr. Carine Prisco Arnoni

Objective: Platelet refractoriness is addressed, emphasizing its definitions, causes and management strategies. Examples of diagnosis, identification of compatible donors and clinical outcomes of refractory patients are illustrated. Speaker: Juliana Pires Marafon Franz, MD, PhD

Objective: The use of molecular typing of blood group genes as a diagnostic tool and its application in transfusion medicine. It covers the characteristics of erythrocyte antigens, advances in genotyping and sequencing techniques, offering greater precision in the identification of rare variants and molecular compatibility, clinical cases and applications, highlighting the importance of personalized medicine in the transfusion context.

Speaker: Lilian Castilho, PhD

Objective: To review the impact of the use of the Microarray BeadChip (Immucor) in the identification of variants of the RHD and RHCE genes, highlighting its application in immunohematology and transfusion. The Microarray BeadChip as an efficient, sensitive and essential tool for solving complex immunohematology problems.

Speaker: Tamires Delfino dos Santos

Objective: To present the isohemagglutinin test, addressing its validation and impact on platelet concentrate transfusions. It includes a case of hemolytic reaction associated with incompatible transfusions, highlighting the importance of preventive measures. It presents data on critical titrations, methodologies and guidelines for minimizing risks in transfusions. Speaker: Ana Paula Rocha Diniz Zanelli

Objective: To review the use of special reagents in immunohematology to solve complex clinical cases such as ABO and RhD group discrepancies, the presence of irregular antibodies and autoimmune hemolytic anemia (AHAI), which require complementary diagnostic techniques. List the benefits of special reagents in facilitating laboratory work, standardizing processes, improving diagnostic accuracy and increasing transfusion safety.

Webinar Objectivo:

El uso de la genotipificación como herramienta para resolver casos complejos de inmunohematología. Incluye estudios de caso, como uno de un paciente con anemia de células falciformes que presentó dificultad respiratoria y múltiples complicaciones transfusionales. Se detalla el análisis de genotipos para identificar anticuerpos y antígenos relevantes, así como estrategias específicas como el uso de células seleccionadas y técnicas avanzadas para confirmar la especificidad de los anticuerpos y optimizar las transfusiones.

Oradora: Dra. Paula A. Gaviria García

Webinar Objectives: 

• Explore the basics of  HLA, HPA and tools available
• Platelet Crossmatching
• Approach to a case of Platelet Refractoriness

Speakers: Dr Mohit Chowdhry, Sr. consultant & HEAD - Department of transfusion medicine, molecular biology and transplant immunology ; Indraprastha Apollo hospital, New Delhi

Webinar Objectives: 

• MFIs between vendors are not comparable and therefore cutoffs based on the same MFI value cannot be applied to both
• Aim is to generate a model to determine how MFI values from different vendors behave
• Establish data-driven, comparable, vendor-specific cutoffs for analyzing big data sets

Speakers: Gonca Karahan, Post Doctoral Researcher, Department of Immunology, Leiden University Medical Center, Netherlands

Webinar Objectives: 

• What is CAR-T, CAR-T current indications and future directions
• The patient journey through CAR T-cell therapy and complications associated with CAR-T

Speakers: Ceri Jones Haematology Consultant, University Hospital of Wales, Emily John Lead CAR-T Clinical Nurse Specialist, University Hospital of Wales

Webinar Objectives: 

Webinar Objectives: 

Webinar Objectives: 

Webinar Objectives: 

• Outlining some of the current Iron and PBM activities being used in the UK
• Highlighting differences in practice when managing paediatrics & adults

Speakers: Dr Paolo Polzella and Dr Anne Kelly

Webinar Objectives: 

•  Implementation of goal-directed bleeding management:
  • Coagulation changes during bleeding
  • What is goal directed bleeding management
  • Why bring in viscoelastic testing
  • How do you introduce it
  • Is it effective – impact on blood product usage

Speakers: Kate Maynard, Dr Sam Hird, Dr Amrit Dhadda and Prof Rachel Collis

Webinar Objectives: 

•  Shared case studies are a learning opportunity for all. Our subject matter experts will be reviewing HLA antibody analysis on the MATCH IT! Antibody software using informative case studies and software tools.

Speakers: Erik Chatroop, MS, MLS(ASCP)^CM, CHS(ACHI) and Lindsay Murdock, MS, CHT(ABHI), MLS(ASCP)^CM

Webinar Summary: 

Using informative case studies and software tools, our subject matter experts will be reviewing HLA typing. Learn more about what makes MIA FORA NGS software and assays unique.

Speakers: Amelia Chen

Webinar Summary: 

Our subject matter experts will be reviewing HLA antibody analysis on MATCH IT! Antibody software using informative case studies, software tools, and interactive polls.

Speakers: K. Kelly Cousins, BS, CHT (ABHI) and Jean L. Heneghan, MS, CHS(ABHI)

Webinar Objectives: 

• Writing procedures and work instructions
• Quality managements
• Standards of laboratory practice
• Test performance appraisal and validation

Speaker: Dr. Marco Andreani Ph.D. ; Dr. Walter Herczyk CHS(ACHI) MT (ASCP)

Webinar Objectives:

• Organ transplantation
• Allogeneic hematopoietic stem cell transplantation
• Single donor platelet transfusion for alloimmunized patients
• HLA and disease associations

Speaker: Dr. Sunil Daga MBBS MRCP PhD

Objetivos del seminario web:

• Se discutira y revisara como resolver casos complejos e intermedios de aloanticuerpos contra células rojas y plaquetas usando serologia y metodos moleculares,
• interferencia de tratamientos monoclonales en immunohematologia ,
• donantes raros y poco comunes

Oradora:  Nancy Benitez;  Claribel Resto; MHSc, MT (ASCP) SBB CM   Baldomero Castro, MD   Lynsi Rahorst, MHPE, MLS(ASCP)SBB Dr.Fernando Martinez, MD, MScP; Section Head and Medical Director Anderson Cancer Center.   Dr. Hector Baptista MD   Marisela Marchan, MSTM , MT (ASCP)SBB

Webinar Objectives 

• Clinical Histocompatibility Testing for Xenotransplantation: What We Have and What We Need
• Post-transplant Rejection Diagnostics:  a Bermuda Triangle between HLA Specialists, Nephrologists and Pathologists
• The Next Steps in the Routine Diagnostic Implementation of Non-HLA Antibody Testing
• The Role of HLA and Non-HLA Antibody Burden in the Outcome of Pediatric Kidney Transplantation

Speakers: Sam Ho, Maarten Naesens, Carrie Butler, Patrizia Comoli

Webinar Objectives 

• Unlocking the Power of PIRCHE-II: Exploring the Role of HLA Mismatch in Risk Stratification
• The Use of PIRCHE in Stem Cell Transplantation
• The HLA Eplet Registry. New Design, New Tools and Future Directions
• Shaping the NEXT GENERATION of MATCH IT!^® Software

Speakers: Bethany Dale, Meenakshi Singh, Massimo Mangolia, Alisa Rantanen

Webinar Objectives

• Best Practices for Antibody Testing
• Canadian Genome Project
• Virtual Crossmatch: How and Why?, US Perspective
• Virtual Crossmatch: How and Why?, Eurotransplant
• Virtual Crossmatch:  How and Why, Down Under

Speakers: Annette Jackson, Karen Sherwood, Paul Keown, Luis Hidalgo, Cynthia Kramer, Rhonda Holdsworth

Obiettivi el corso:

• Nomenclatura
• HLAi & UKLKSS
• Allocazione
• Post-trapianto

Oratore: Miriam Manook, MBChB, MRCS (Ed), PhD

Obiettivi el corso:

• Getione delle liste di lavoro
• Gestione dei risultati
• Gestione dei report
• Modulo Panel ID
• Gestione degli archivi

Oratore: Gianni Giuffrè

Obiettivi del webinar:

• Trasfusioni di globuli rossi nei neonati
• Trasfusioni di piastrine nei neonati

Oratore: Martha Sola-Visner. MD.

Obiettivi del webinar:
• Definire risultati di laboratorio di coagulazione normali e anormali.
• Correlare i risultati di laboratorio con segni e sintomi clinici comuni nel paziente.
• Discriminare i risultati di laboratorio delle piastrine normali e anormali.
• Applicare i risultati della coagulazione e dell'ematologia in esempi di casi di studio incentrati sulla gestione di trasfusioni di emoderivati appropriate o interventi medici.

Oratore: Jayanna Slayten, MS, MT(ASCP)SBB^CM

Obiettivi el corso:

• Effetto della variazione del segnale peptide HLA di classe I sull'espressione di HLA-E

Oratore: Miriam Manook, MBChB, MRCS (Ed), PhD

Webinar Objectives: 

• Sensitivity of antibody detection assays
• Epitope of structure of HLA gene products
• Relationships of HLA epitopes and sequence
•  Analysis of HLA antibodies in patients
• Dynamics of HLA antibody production

Speaker: Tiffany Bratton, PhD

Objetivos do seminário web:

• Isohemaglutininas e a Transfusao de Plaquetas
• validação do crossmatch plaquetario por fase sólida para manejo da Refratariedade Plaquetaria.
• inovações para a identificaçao de anticorpos de especificidade não determinada e as técnicas moleculares na determinação de alelos de grupos.

Orador: Jayanna Slayten, MS, MT(ASCP)SBBCM

Webinar Objectives: 

• Complement-dependent cytotoxicity assay,
  flow cytometry, & ELISA
• Complement and antiglobulin reagents Molecular genetics

Speaker: Michael D. Gautreaux, PhD, FACHI

Webinar Objectives: 

• HLA complex & nomenclature
• Genetics of the HLA complex
• Physical and antigenic structure & functioning of HLA gene products

Speaker: Gizem Turmer, MD

Objectif du cours:

• Nomanclature
• HLAi & UKLKSS
• Allocation
• Post-transplantation

Orateur: Miriam Manook, MBChB, MRCS (Ed), PhD

Objectif du webinaire

• Effet de la variation du peptide signal HLA de classe I sur l'expression HLA-E

Speaker: Dr. Mary Carrington, Center for Cancer Research, Bethesda, ML

Objectif du webinaire:
• Définir les résultats normaux et anormaux du laboratoire de coagulation.
• Corréler les résultats de laboratoire avec les signes et symptômes cliniques courants chez le patient.
• Distinguer les résultats normaux et anormaux du laboratoire de plaquettes.
• Appliquer les résultats de la coagulation et de l'hématologie dans des exemples d'études de cas sur la provision de transfusion appropriée de produits sanguins ou d'une intervention médicale.

Orateur: Jayanna Slayten, MS, MT(ASCP)SBB^CM

Objectif du webinaire:

• Gestion des listes de travail
• Fgestion des résultats
• Gestion des rapports
• Module Panel ID
• Gestion des archives

Orateur: Gianni Giuffrè

Webinar Objective:

• LEARNing is fun in a new way when you complete this virtual intensive, transfusion medicine investigation.

Webinar Objectives:

• LEARNing is fun in a new way when you complete this virtual intensive, transplant medicine investigation.