Webinar Objectives: 

• Introduction & importance of Antibody screening
• Clinical relevance
• Case-based Learning : Insight from experience

Speakers: Dr Sudha Ranganathan, Consultant & HEAD - Transfusion Medicine Apollo Hospital, Hyderabad- India 

Objective: This article discusses Platelet Crossmatch, an essential method for platelet compatibility in refractory patients. The technique is used to identify compatible platelets in alloimmunized patients. The method is based on the solid phase (Capture-P®), which detects IgG antibodies against platelets. It highlights the importance of standardized methods to improve the management of refractory patients and optimize clinical results. Speaker: Cristina Altobeli de Brito

Objective: To address RhD variants and the importance of their serological detection to guide molecular investigations. It explains how mutations such as SNPs, deletions and gene conversions result in variants such as partial D, weak D and Del. These alterations can cause differences in epitope expression and antisera reactivity. It includes examples of discrepancies in RhD typing and molecular characterization, such as a donor with a CDe haplotype and a patient with myelodysplastic syndrome. The importance of standardized methods and knowledge of variants for safe transfusion practices and accurate diagnoses is reinforced. Speaker: Dr. Tatiane de Paula Vendrame

Objective: A study of RhCE variants is presented, covering detection, classification and clinical significance. RhCE variants are detailed as variant proteins due to genetic polymorphisms, which can affect the expression of RhCE antigens such as C, c, E and e. Weakened expression, partial antigens and the absence of high-frequency antigens are mentioned. The need for greater knowledge of these variants to improve transfusions and blood compatibility in chronically transfused patients is emphasized. Speaker: Dr. Carine Prisco Arnoni

Objective: Platelet refractoriness is addressed, emphasizing its definitions, causes and management strategies. Examples of diagnosis, identification of compatible donors and clinical outcomes of refractory patients are illustrated. Speaker: Juliana Pires Marafon Franz, MD, PhD

Objectives: The implementation of the Type and Screen (TAS) protocol in the blood bank of an emergency center in Lima, Peru, is analyzed, highlighting its usefulness for detecting irregular antibodies (Ac) in safe transfusions. The main points are: The Type and Screen Protocol is a method that combines blood typing (ABO/RhD) with irregular antibody screening to predict compatibility before transfusion in a prospective study between 2016 and 2022, where several types of antibodies were detected, the most common being Anti-E, Anti-Dia and autoantibodies. Solid-phase Capture (Immucor) technology was key to identifying these antibodies. Lissette Fernandez

Objective: To review genomic concepts of erythrocyte antigens. The use of immunohematology when patients have been recently transfused, when patients have some antibody to a high or low frequency antigen but no commercial antibody is available, for the management of patients receiving monoclonal therapies, and in prenatal testing to predict hemolytic disease of the newborn. It focuses on the identification of donors with rare blood.

Speaker: Celina Montemayor MD, PhD

Objectives: The importance of titration of natural anti-A and anti-B antibodies in platelet donors is described, highlighting their relevance in reducing post-transfusion hemolytic reactions.

Orador: Lic. Gisel Aguilar Ocaña

Objective: The management of patients refractory to platelet transfusions is addressed, highlighting key concepts, etiology and therapeutic strategies. An example of management in a patient with acute myeloid leukemia refractory to transfusions due to anti-HLA antibodies is illustrated, highlighting the importance of specific tests and personalized transfusions. Correct identification of the cause and personalized management are essential to improve outcomes in refractory patients and the need for specific protocols and adequate resources to address this complex condition is emphasized.

Speaker: Mayrin Correa Medina, MD, Ph.D.

Objective: Key concepts, etiology and therapeutic strategies for the management of patients refractory to platelet transfusions are highlighted. An example of management in a patient with acute myeloid leukemia refractory to transfusions due to anti-HLA antibodies is illustrated, highlighting the importance of specific tests and personalized transfusions. The need for specific protocols and adequate resources to address this complex condition is emphasized. Correct identification of the cause and personalized management are essential to improve outcomes in refractory patients.

Speaker: Fernando Martinez

Objectives: The paper addresses hemolytic disease of the newborn (HDNB) caused by maternal alloimmunization, exploring various clinical, serological and epidemiological aspects. The importance of population genetics and health systems in the management of this neonatal disease is highlighted. EHRN is preventable and treatable through early diagnosis, proper management of blood incompatibility and immunization programs.

Speaker: Dr. Hector Baptista Gonzalez, MD

Objectives: To recognize the serologic presentations of Autoimmune Hemolytic Anemia (AHA). To understand the limitations of abbreviated diagnostic assays. Differentiate cold autoantibody reactivity. The study underlines the importance of detailed testing to correctly characterize autoantibodies and properly manage the condition.

Speaker: Lauro E. Guerra, Jr. MLS(ASCP)SBBCM

Objective: The use of molecular typing of blood group genes as a diagnostic tool and its application in transfusion medicine. It covers the characteristics of erythrocyte antigens, advances in genotyping and sequencing techniques, offering greater precision in the identification of rare variants and molecular compatibility, clinical cases and applications, highlighting the importance of personalized medicine in the transfusion context.

Speaker: Lilian Castilho, PhD

Objective: To review the impact of the use of the Microarray BeadChip (Immucor) in the identification of variants of the RHD and RHCE genes, highlighting its application in immunohematology and transfusion. The Microarray BeadChip as an efficient, sensitive and essential tool for solving complex immunohematology problems.

Speaker: Tamires Delfino dos Santos

Objective: To present the isohemagglutinin test, addressing its validation and impact on platelet concentrate transfusions. It includes a case of hemolytic reaction associated with incompatible transfusions, highlighting the importance of preventive measures. It presents data on critical titrations, methodologies and guidelines for minimizing risks in transfusions. Speaker: Ana Paula Rocha Diniz Zanelli

Objective: To review the use of special reagents in immunohematology to solve complex clinical cases such as ABO and RhD group discrepancies, the presence of irregular antibodies and autoimmune hemolytic anemia (AHAI), which require complementary diagnostic techniques. List the benefits of special reagents in facilitating laboratory work, standardizing processes, improving diagnostic accuracy and increasing transfusion safety.

Webinar Objetivos:

Describe las las discrepancias y subgrupos en el sistema sanguíneo ABO, diferenciando entre: Discrepancias ABO cuando hay inconsistencias entre las pruebas de agrupamiento directo e inverso, o entre resultados pasados y actuales. Subgrupos ABO, Representan variantes del sistema ABO con niveles reducidos de antígenos en los glóbulos rojos. Se presentan ejemplos donde discrepancias y anticuerpos inesperados, como anti-A1, resultaron en reacciones transfusionales severas, subrayando la importancia de la tipificación precisa y el manejo adecuado. Importancia clínica, Se enfatiza la necesidad de protocolos robustos y herramientas avanzadas para garantizar la seguridad del paciente.

Orador: Dr. Baldomero Castro

Webinar Objetivos:

Describir la importancia de los donantes de reactivos en la fabricación de reactivos de glóbulos rojos utilizados para pruebas serológicas en laboratorios y bancos de sangre. Explica cómo estos donantes, seleccionados por su fenotipo específico, garantizan la disponibilidad de unidades necesarias para detectar anticuerpos y tipificar sangre de manera precisa. Se aborda los desafíos como la competencia con necesidades transfusionales y requisitos regulatorios internacionales, además de destacar configuraciones como las pruebas Capture^® para detección de anticuerpos. También subraya que los donantes de reactivos permiten estabilidad en la producción y evitan retrasos en el suministro de productos esenciales.

Orador: Germán Núñez, MBA

Webinar Objetivo:

Describir las causas y aplicaciones de la prueba de antiglobulina directa (Coombs directo), destacando su relevancia en diagnósticos como anemia hemolítica autoinmune, reacciones transfusionales y enfermedad hemolítica del recién nacido. Incluye ejemplos prácticos para analizar casos, como discrepancias ABO madre-bebé, y destaca técnicas como la elución para identificar anticuerpos específicos adheridos a glóbulos rojos.

Orador: Nancy Benitez, MHS(ASCP) SBB

Webinar Objectivo:

El uso de la genotipificación como herramienta para resolver casos complejos de inmunohematología. Incluye estudios de caso, como uno de un paciente con anemia de células falciformes que presentó dificultad respiratoria y múltiples complicaciones transfusionales. Se detalla el análisis de genotipos para identificar anticuerpos y antígenos relevantes, así como estrategias específicas como el uso de células seleccionadas y técnicas avanzadas para confirmar la especificidad de los anticuerpos y optimizar las transfusiones.

Oradora: Dra. Paula A. Gaviria García

Webinar Objectives: 

• Join us to learn the best practices for writing abstracts and posters as well as how to do your study or project justice when presenting at a conference.
• Learn what is and isn't an abstract, and what should an abstract contain.
• Hear about the importance of posters in academic and professional settings.
• Learn tips for writing abstracts, creating effective posters, including structure, design and the pitfalls to avoid.

Speakers: Richard Haggas, Director, UK NEQAS Blood Transfusion Laboratory Practice and Alison Hines, Scientific and Clinical Training and Education Manager, NHSBT

Webinar Objectives: 

• Explore the basics of  HLA, HPA and tools available
• Platelet Crossmatching
• Approach to a case of Platelet Refractoriness

Speakers: Dr Mohit Chowdhry, Sr. consultant & HEAD - Department of transfusion medicine, molecular biology and transplant immunology ; Indraprastha Apollo hospital, New Delhi

Webinar Objectives: 

• MFIs between vendors are not comparable and therefore cutoffs based on the same MFI value cannot be applied to both
• Aim is to generate a model to determine how MFI values from different vendors behave
• Establish data-driven, comparable, vendor-specific cutoffs for analyzing big data sets

Speakers: Gonca Karahan, Post Doctoral Researcher, Department of Immunology, Leiden University Medical Center, Netherlands

Webinar Objectives: 

• What is CAR-T, CAR-T current indications and future directions
• The patient journey through CAR T-cell therapy and complications associated with CAR-T

Speakers: Ceri Jones Haematology Consultant, University Hospital of Wales, Emily John Lead CAR-T Clinical Nurse Specialist, University Hospital of Wales

Webinar Objectives: 

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Webinar Objectives: 

Webinar Objectives: 

• Outlining some of the current Iron and PBM activities being used in the UK
• Highlighting differences in practice when managing paediatrics & adults

Speakers: Dr Paolo Polzella and Dr Anne Kelly

Webinar Objectives: 

•  Implementation of goal-directed bleeding management:
  • Coagulation changes during bleeding
  • What is goal directed bleeding management
  • Why bring in viscoelastic testing
  • How do you introduce it
  • Is it effective – impact on blood product usage

Speakers: Kate Maynard, Dr Sam Hird, Dr Amrit Dhadda and Prof Rachel Collis

Webinar Objectives: 

•  Shared case studies are a learning opportunity for all. Our subject matter experts will be reviewing HLA antibody analysis on the MATCH IT! Antibody software using informative case studies and software tools.

Speakers: Erik Chatroop, MS, MLS(ASCP)^CM, CHS(ACHI) and Lindsay Murdock, MS, CHT(ABHI), MLS(ASCP)^CM

Webinar Summary: 

Using informative case studies and software tools, our subject matter experts will be reviewing HLA typing. Learn more about what makes MIA FORA NGS software and assays unique.

Speakers: Amelia Chen

Webinar Summary: 

Our subject matter experts will be reviewing HLA antibody analysis on MATCH IT! Antibody software using informative case studies, software tools, and interactive polls.

Speakers: K. Kelly Cousins, BS, CHT (ABHI) and Jean L. Heneghan, MS, CHS(ABHI)

Webinar Objectives: 

• HLA antibody analysis on MATCH IT! Antibody v1.5.1 software using informative case studies and software tools

Speakers: Lindsay Murdock, MS, CHT(ABHI), MLS(ASCP) ^CM  and Erik Chatroop, MS, MLS(ASCP)^CM, CHS(ACHI)

Webinar Objectives: 

• Writing procedures and work instructions
• Quality managements
• Standards of laboratory practice
• Test performance appraisal and validation

Speaker: Dr. Marco Andreani Ph.D. ; Dr. Walter Herczyk CHS(ACHI) MT (ASCP)

Webinar Objectives:

• Organ transplantation
• Allogeneic hematopoietic stem cell transplantation
• Single donor platelet transfusion for alloimmunized patients
• HLA and disease associations

Speaker: Dr. Sunil Daga MBBS MRCP PhD

Objetivos del seminario web:

• Se discutira y revisara como resolver casos complejos e intermedios de aloanticuerpos contra células rojas y plaquetas usando serologia y metodos moleculares,
• interferencia de tratamientos monoclonales en immunohematologia ,
• donantes raros y poco comunes

Oradora:  Nancy Benitez;  Claribel Resto; MHSc, MT (ASCP) SBB CM   Baldomero Castro, MD   Lynsi Rahorst, MHPE, MLS(ASCP)SBB Dr.Fernando Martinez, MD, MScP; Section Head and Medical Director Anderson Cancer Center.   Dr. Hector Baptista MD   Marisela Marchan, MSTM , MT (ASCP)SBB

Webinar Objectives 

• Clinical Histocompatibility Testing for Xenotransplantation: What We Have and What We Need
• Post-transplant Rejection Diagnostics:  a Bermuda Triangle between HLA Specialists, Nephrologists and Pathologists
• The Next Steps in the Routine Diagnostic Implementation of Non-HLA Antibody Testing
• The Role of HLA and Non-HLA Antibody Burden in the Outcome of Pediatric Kidney Transplantation

Speakers: Sam Ho, Maarten Naesens, Carrie Butler, Patrizia Comoli

Webinar Objectives 

• Unlocking the Power of PIRCHE-II: Exploring the Role of HLA Mismatch in Risk Stratification
• The Use of PIRCHE in Stem Cell Transplantation
• The HLA Eplet Registry. New Design, New Tools and Future Directions
• Shaping the NEXT GENERATION of MATCH IT!^® Software

Speakers: Bethany Dale, Meenakshi Singh, Massimo Mangolia, Alisa Rantanen

Webinar Objectives

• Best Practices for Antibody Testing
• Canadian Genome Project
• Virtual Crossmatch: How and Why?, US Perspective
• Virtual Crossmatch: How and Why?, Eurotransplant
• Virtual Crossmatch:  How and Why, Down Under

Speakers: Annette Jackson, Karen Sherwood, Paul Keown, Luis Hidalgo, Cynthia Kramer, Rhonda Holdsworth

Obiettivi el corso:

• Nomenclatura
• HLAi & UKLKSS
• Allocazione
• Post-trapianto

Oratore: Miriam Manook, MBChB, MRCS (Ed), PhD

Obiettivi el corso:

• Getione delle liste di lavoro
• Gestione dei risultati
• Gestione dei report
• Modulo Panel ID
• Gestione degli archivi

Oratore: Gianni Giuffrè

Obiettivi del webinar:

• Trasfusioni di globuli rossi nei neonati
• Trasfusioni di piastrine nei neonati

Oratore: Martha Sola-Visner. MD.

Obiettivi del webinar:
• Definire risultati di laboratorio di coagulazione normali e anormali.
• Correlare i risultati di laboratorio con segni e sintomi clinici comuni nel paziente.
• Discriminare i risultati di laboratorio delle piastrine normali e anormali.
• Applicare i risultati della coagulazione e dell'ematologia in esempi di casi di studio incentrati sulla gestione di trasfusioni di emoderivati appropriate o interventi medici.

Oratore: Jayanna Slayten, MS, MT(ASCP)SBB^CM

Obiettivi el corso:

• Effetto della variazione del segnale peptide HLA di classe I sull'espressione di HLA-E

Oratore: Miriam Manook, MBChB, MRCS (Ed), PhD

Webinar Objectives: 

• Sensitivity of antibody detection assays
• Epitope of structure of HLA gene products
• Relationships of HLA epitopes and sequence
•  Analysis of HLA antibodies in patients
• Dynamics of HLA antibody production

Speaker: Tiffany Bratton, PhD

Objetivos do seminário web:

• Isohemaglutininas e a Transfusao de Plaquetas
• validação do crossmatch plaquetario por fase sólida para manejo da Refratariedade Plaquetaria.
• inovações para a identificaçao de anticorpos de especificidade não determinada e as técnicas moleculares na determinação de alelos de grupos.

Orador: Jayanna Slayten, MS, MT(ASCP)SBBCM

Webinar Objectives: 

• Complement-dependent cytotoxicity assay,
  flow cytometry, & ELISA
• Complement and antiglobulin reagents Molecular genetics

Speaker: Michael D. Gautreaux, PhD, FACHI

Webinar Objectives: 

• HLA complex & nomenclature
• Genetics of the HLA complex
• Physical and antigenic structure & functioning of HLA gene products

Speaker: Gizem Turmer, MD

Objectif du cours:

• Nomanclature
• HLAi & UKLKSS
• Allocation
• Post-transplantation

Orateur: Miriam Manook, MBChB, MRCS (Ed), PhD

Objectif du webinaire

• Effet de la variation du peptide signal HLA de classe I sur l'expression HLA-E

Speaker: Dr. Mary Carrington, Center for Cancer Research, Bethesda, ML